P-48: Nicotine Alters Both Somatic and Germ Cells in Adult Mouse Testis

Authors

Abstract:

Background: Nicotine as a toxic agent in cigarette has detrimental effects on reproduction. The aim of this study was to evaluate effects of of nicotine on germ and somatic cells in adult mouse testis. Materials and Methods: Male mice were divided into four groups. Group A or controls, groups of B, C and D were treated with nicotine intraperitoneally in doses of 0.1, 0.2 and 0.4 mg/100 g body weight once daily for 14 days respectively. Evaluations were made by Transmission Electron Microscopy (TEM), determining of mitotic activity of cells by KI-67 staining, and ELISA for assay of serum testosterone levels Results: TEM revealed alterations both in germ and somatic cells in nicotine treated groups. Nicotine in groups of C and D caused to deformity in nucleus, reduced numbers of lipid droplets and mitochondria in leydig cells. Myoid cells had deformed and irregular nucleus in groups of C and D. the signs of apoptosis were recognizable in primary spermatocytes and spermatids in groups of C and D. In group D some sertoli cells had apoptotic bodies. Administration of nicotine in group D, significantly reduced KI-67 index compared with control group. Serum testosterone levels and maturity of spermatogenesis were significantly reduced in groups of C and D in compare with controls. Conclusion: This study indicates that nicotine can affect both on germ and somatic cells in testis. Nicotine induces apoptosis and reduces mitotic activity in male germ cells as a dose dependent manner.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

P-10: Proliferative Activity of Adult Mouse Male Germ Cells Following Administration of Graded Doses of Nicotine

Background: Nicotine is considered as one of the most agents in cigarette smoking and has side effects on many organs and body systems such as reproductive tract. Nicotine can induce sub fertility or infertility both in males and females. The aim of this study was to evaluate the proliferative activity of adult mouse male germ cells following treatment with different doses of nicotine. Material...

full text

P-46: Proliferative Activity of Follicular Cells in Adult Mouse Ovary Following Administration of Exogenous Nicotine

Background: Nicotine can induce sub fertility and infertility in females. The aim of this study was to determine the effect of nicotine on follicular cells proliferative activity in mouse ovary. Materials and Methods: Female adult NMRI mice were divided into two groups. Animals in control group received vehicle, however in experimental group, animals were treated with 0.4mg/kg of nicotine for 1...

full text

Evaluation of Proliferative Activity of Adult Mouse Male Germ Cells Following Administration of Different Doses of Nicotine

Purpose: Nicotine can induce sub fertility or infertility both in males and females. The aim of this study is to evaluate the proliferative activity of adult mouse male germ cells following treatment with different doses of nicotine.Materials and Methods: Adult male NMRI mice were divided into four groups. The control group received normal saline and the other animals in groups of 2, 3 and 4 we...

full text

Buserelin Inhibits Apoptosis in Male Germ Cells Induced by Busulfan in Mouse Testis

Purpose: The aim of this study was to investigate the effect of buserelin on apoptosis of male germ cells induced by busulfan in adult male mice. Materials and Methods: Male adult NMRI mice were divided into four group of eight each. Group 1 (control) administered PBS for 21 days subcutaneously, group 2 given 0.4 mg buserelin for 21 days subcutaneously, group 3 given single dose of 30 mg/kg bus...

full text

Survival of glucose phosphate isomerase null somatic cells and germ cells in adult mouse chimaeras

The mouse Gpi1 gene encodes the glycolytic enzyme glucose phosphate isomerase. Homozygous Gpi1(-/-) null mouse embryos die but a previous study showed that some homozygous Gpi1(-/-) null cells survived when combined with wild-type cells in fetal chimaeras. One adult female Gpi1(-/-)↔Gpi1(c/c) chimaera with functional Gpi1(-/-) null oocytes was also identified in a preliminary study. The aims we...

full text

P-74: Crocin Repopulate The Leydig and Sertoli Cells in Testis of Cyclophosphamide Treated Adult Mice

Background: Increases in the survival rate of men treated with chemotherapeutic drugs and their desire to have children precipitate concerns about the effects of these drugs on germ cells. This study performed to evaluation of protective effects of crocin in oxidative stress induced by cyclophosphamide (CP) on testis. Materials and Methods: Three groups (6 mice in each) of adult mice were used....

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 6  issue 2

pages  -

publication date 2012-09-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023